Gene expression profiling of blood of Ebola virus disease survivors and Healthy donors by Next Generation Sequencing

Clinical follow-up of Ebola virus disease (EVD) survivors revealed a persistence of clinical symptoms and higher risk of mortality. Long-term analyses of the immune and inflammatory profiles of EVD survivors are currently lacking. Here, we evaluate gene expression profiles in 26 Guinean Ebola virus disease survivors (EBOV_S) and 33 Healthy donnors (HD). We show a persistent increase of several biomarkers of inflammation, immune activation and gut tissue damage in EBOV_S compared to healthy donors living in the same area. Gene expression profiling in blood revealed a significant enrichment in genes associated with antiviral responses in EBOV_S. Overall design: Analysis of whole-blood gene expression profiles of 26 Ebola virus disease survivors and 33 Healthy donnors

埃博拉病毒病（Ebola Virus Disease, EVD）幸存者的临床随访研究显示，其临床症状持续存在，且死亡风险更高。目前针对EVD幸存者的免疫与炎症特征的长期分析仍较为匮乏。本研究对26名来自几内亚的埃博拉病毒病幸存者（EBOV_S）及33名健康供体（Healthy donors, HD）的基因表达谱进行了分析。结果表明，与同地区健康供体相比，EBOV_S群体中多种炎症、免疫激活及肠道组织损伤相关生物标志物的水平持续升高。血液基因表达谱分析显示，EBOV_S群体中与抗病毒应答相关的基因显著富集。研究整体设计：对26名埃博拉病毒病幸存者及33名健康供体的全血基因表达谱进行分析。