Expression profiling of mouse livers with inducible over-expression of microRNA Mir483

In this study we generated mice with inducible over-expression of microRNA Mir483, using a transgene inserted at the Rosa26 locus. Whole transcriptome analysis was performed by RNA-seq in livers collected from 15-week old adult mice (over-expressors and controls). Overall design: We performed RNA-seq analysis in 15-week old mouse livers of adult females with inducible over-expression of microRNA Mir483 (n=6 biological replicates) and littermate controls (n=6 biological replicates)

本研究通过在Rosa26基因座插入转基因的方式，构建了可诱导过表达微小RNA（microRNA）Mir483的小鼠模型。本研究采用RNA测序（RNA-seq）技术，对15周龄成年小鼠采集的肝脏组织开展全转录组分析，实验分组涵盖过表达组与对照组。
实验整体设计：本研究针对15周龄成年雌性小鼠的肝脏组织进行RNA测序分析，其中微小RNA Mir483可诱导过表达组设置6例生物学重复，同窝对照组同样设置6例生物学重复。