SAPrIm 2.0: A semi-automated protocol for mid-throughput soluble HLA immunopeptidomics

Human leukocyte antigen (HLA) molecules are pivotal in guiding human adaptive immune responses through their presentation of peptide ligands, collectively known as the immunopeptidome. This process is central to the development of cancer immunotherapies, such as vaccines and T-cell therapies. Profiling the immunopeptidome from plasma and other biofluids has gained increasing traction, as it offers a minimally invasive approach for monitoring disease states and immune responses toward cancer therapy. Here we present the second iteration of SAPrIm, a refined immunopeptidomics tool optimized for soluble HLA analysis. It can process up to 12 samples per batch within a day. This workflow is positioned to advance the field of immunopeptidomics by enabling efficient plasma-based comparative analyses and mid-size cohort studies.

人类白细胞抗原（Human leukocyte antigen，HLA）通过呈递统称为免疫肽组（immunopeptidome）的肽配体调控人类适应性免疫应答，是该过程的关键调控分子。这一过程是癌症免疫疗法（如疫苗与T细胞疗法）研发的核心环节。从血浆及其他生物体液中开展免疫肽组谱型分析的研究愈发受到关注，因其可提供一种微创手段，用于监测疾病状态以及癌症治疗过程中的免疫应答情况。本文介绍了SAPrIm的第二个迭代版本，这是一款针对可溶性HLA分析优化的改良型免疫肽组学（immunopeptidomics）工具。该工具单日每批次可处理至多12份样本。此实验流程可助力开展高效的血浆源性比较分析与中型队列研究，进而推动免疫肽组学领域的发展。