Global RNA half lives in cyanobacteria

We calculated global RNA half lives for all genes in the cyanobacteria Synechococcus sp. strain PCC 7002. Samples from three replicates of the WT strain were taken before and following the addition of the antibiotic rifampicin to stop nascent transcription. RNA spike-ins were added for normalization and using the number of RNA-sequencing reads we were able to calculate half lives for genes, transcripts, and for each position on the genome. Samples were taken from three replicates of WT PCC 7002 (labeled 2,3,and 4). Samples taken before the addition of rifampicin were called '0 minutes' and labeled with 'A'. Subsequent timepoints (0.5 minutes, 1 minute, 2.5 minutes, 5 minutes, 7.5 minutes, and 10 minutes) were labeled respectively B, C, D, E, F, and G. Three replicates were taken with 7 timepoints each leading to 21 samples total.

我们针对蓝细菌聚球藻（Synechococcus sp. strain PCC 7002）的全部基因，计算了其全局RNA半衰期。我们从野生型（Wild Type, WT）菌株的3个生物学重复中，分别在添加抗生素利福平（rifampicin）以阻断新生转录前后采集样本；通过加入RNA内参（RNA spike-in）完成标准化，并结合RNA测序读段（RNA-sequencing reads）的计数结果，得以计算得到基因、转录本以及基因组上每个位点的RNA半衰期。本次实验的样本取自3个生物学重复的野生型PCC 7002菌株，分别标记为2、3和4；利福平添加前采集的样本记为"0分钟"组，标记为"A"；后续的时间点样本（0.5分钟、1分钟、2.5分钟、5分钟、7.5分钟及10分钟）依次标记为B、C、D、E、F、G。每个时间点设置3个生物学重复，共计7个时间点，最终总计得到21份样本。