Eradiation of leukemia by effective reprogramming-associated cell elimination (ERACE)

We used microarrays to evaluate the global programming of gene expression after Dox inducible Yamanaka factors ectopic expression and identified distinct classes of genes during this biological process in vivo. Leukemia cells were sorted from distinct groups at different time points. Microarray was performed at GMINIX in Shanghai. To investigate the molecular mechanism for the elimination of leukemia cells after Dox inducible Yamanaka factors (Oct4,Sox2,Klf4,c-Myc) expression, we sorted GFP+ MLL-AF9 acute myeloid leukemia cells (AML) from leukemic mice and normal Lin-ckit+sca1+ cells (LKS) from 4F transgenic mice. We then performed microarray analysis of these samples before and after Dox-induced OSKM expression or treatment with reprogramming compound cocktail (VPA, CHIR, 616452, TCP) at 24h in vitro.

我们采用基因芯片（microarrays）技术，评估了多西环素（Dox）诱导的山中因子（Yamanaka factors）异位表达后基因表达的全局调控程序，并在该体内生物学过程中鉴定出不同类别的基因。
我们在不同时间点从不同实验组中分选了白血病细胞。
本研究的基因芯片检测工作在上海GMINIX完成。
为探究多西环素诱导的山中因子（Oct4、Sox2、Klf4、c-Myc）表达后白血病细胞清除的分子机制，我们分别从白血病小鼠体内分选了GFP阳性的MLL-AF9急性髓系白血病（AML）细胞，并从4F转基因小鼠体内分选了正常Lin⁻cKit⁺Sca1⁺细胞（LKS）。
随后，我们在体外对经多西环素诱导OSKM表达，或用重编程化合物组合（VPA、CHIR、616452、TCP）处理24小时后的上述样本进行了基因芯片分析。