Immunohistochemical analysis of NEFH in esophageal cancer tissue microarray with normal tissue controls (ES804).

Expression level was indicated as -, absent or faint expression; +, moderate expression; ++, expression; +++, strong expression.
NEFH positivity (+) was counted in samples with over moderate expression.
P values from Fisher's exact test performed in normal vs. hyperplasia, adenocarcinoma, squamous cell carcinoma, or metastatic cancer.
*P<0.05 considered significant.

表达水平以分级符号表示：- 代表无表达或微弱表达；+ 代表中等表达；++ 代表阳性表达；+++ 代表强阳性表达。
神经丝蛋白重链（NEFH）阳性（+）的计数对象为中等表达水平以上的样本。
采用费舍尔精确检验，计算正常组织与增生、腺癌、鳞状细胞癌、转移性癌组间的P值。
*P<0.05 被视为具有统计学显著性。