RNA-Seq Data of <i>Ceratocystis fimbriata</i>and <i>Bacillus licheniformis </i>BL06 SFB-1 Interaction for Biological Control in Sweet Potato

This dataset contains RNA-Seq data from <i>Ceratocystis fimbriata</i> spores exposed to the sterile supernatant (CFS) of <i>Bacillus licheniformis</i> BL06 for biological control in sweet potato. The <i>C. fimbriata</i> spore suspension (1 × 10^6 spores/mL) was cultured in a liquid medium (PDA: CFS = 1:1) for 0, 6, 24, and 48 hours at 28°C with shaking at 180 rpm. The time points are categorized as follows: 0 h as the control (CK), 6 h as CFT1, 24 h as CFT2, and 48 h as CFT3. Each treatment was repeated three times. After culture, spores were harvested by centrifugation, washed with sterile water, and sent to Suzhou Panomike Biomedicine Technology Co., Ltd. for RNA extraction, library construction, and sequencing. The raw sequencing data underwent quality control to ensure high-quality results. These data were aligned to the sweet potato genome (GCA_000389695.3) using HISAT2 (v2.0.5), and transcript assembly was performed. Gene expression levels were quantified using FPKM (Fragments Per Kilobase per Million).本数据集包含经地衣芽孢杆菌（Bacillus licheniformis）BL06无菌上清液（CFS）处理的、用于甘薯生物防治的长喙壳菌（Ceratocystis fimbriata）孢子的RNA测序（RNA-Seq）数据。将浓度为1×10^6孢子/mL的长喙壳菌孢子悬浮液接种于PDA与无菌上清液（CFS）体积比为1:1的液体培养基中，于28℃、180 rpm条件下振荡培养，分别设置0、6、24、48小时四个采样时间点。时间点分组如下：0小时为对照组（CK），6小时组记为CFT1，24小时组记为CFT2，48小时组记为CFT3，每组处理设置3次生物学重复。培养结束后，通过离心收集孢子，经无菌水洗涤后，送至苏州帕诺米克生物医药科技有限公司（Suzhou Panomike Biomedicine Technology Co., Ltd.）进行RNA提取、文库构建及测序。原始测序数据先经过质量控制以保障数据质量。使用HISAT2（v2.0.5）将测序数据比对至甘薯参考基因组（GCA_000389695.3），并完成转录本组装。采用FPKM（每百万片段每千碱基片段数，Fragments Per Kilobase per Million）量化基因表达水平。