Sequencing of Saccharomyces cerevisiae strains with tandem amplified GFP and Aeblue genes

We aimed to link cellular fitness to production of a desirable compound in model organism Saccharomyces cerevisiae. A tri-vector system was used to integrate a bacterial mRNA interferase toxin RelE, along with its orthogonal antidote RelB in sequence with the productive gene. Induction of the toxin increases the selection pressure for high antitoxin producing cells, one outcome of which is the recovery of a strain with a high copy number of tandem-repeated antitoxin-gene of interest arrays. The sequences contained within this submission were obtained from yeast produced by this method and were sequenced in the course of its characterisation.

本研究旨在将模式生物酿酒酵母（Saccharomyces cerevisiae）的细胞适应性与目标化合物的生产相关联。研究采用三载体系统，将细菌mRNA干扰酶毒素RelE及其正交解毒剂RelB与生产性基因按序列顺序整合。诱导毒素表达可提升对高解毒剂产生细胞的选择压力，该选择压力的一项直接成果是筛选得到携带高拷贝数目标解毒剂-目标基因串联重复阵列的菌株。本提交所包含的序列均来自通过该方法构建的酵母菌株，并在菌株表征过程中完成测序。