Effects of Heat Stress on Expression of Heat Shock Proteins in the Small Intestine of Wenchang Chicks

ABSTRACT In this study, immunohistochemistry and real-time fluorescent-based quantitative PCR were used to evaluate the expression of heat shock protein (HSP) 60, HSP70, and HSP90 in the small intestine of Wenchang chicks. Compared with the control group (CK), the positive expression of HSP60 and HSP60 mRNA in the heat stress group (HS) were initially higher and subsequently lower in the duodenum (p<0.01). In the HS jejunum, the levels of HSP60 were higher (p<0.01) and the HSP60 mRNA was lower (p<0.05). Whereas the levels of HSP60 in the HS ileum were higher (p<0.05) and then lower (p<0.01), and the HSP60 mRNA was higher (p<0.01). The levels of HSP70 were higher (p<0.01) and the HSP70 mRNA was lower in the duodenum (p<0.05), while the expression of both HSP70 and HSP70 mRNA was higher in the jejunum (p<0.01) and the ileum (p<0.05) of the HS. In the HS duodenum the levels of HSP90 were lower and then higher (p<0.01), and the HSP90 mRNA was higher (p<0.01). The expression of both HSP90 and HSP90 mRNA was higher in the HS jejunum (p<0.05). The levels of HSP90 were lower while the HSP90 mRNA was higher in the HS ileum (p<0.01). These results indicate that heat stress disturbed the expression of HSP60, HSP70, and HSP90 in the small intestine of chicks.

摘要
本研究采用免疫组织化学法（immunohistochemistry）与实时荧光定量聚合酶链式反应（real-time fluorescent-based quantitative PCR），检测文昌雏鸡小肠组织中热休克蛋白（heat shock protein, HSP）60、HSP70及HSP90的表达水平。与对照组（CK）相比，热应激组（HS）雏鸡十二指肠组织中HSP60蛋白及HSP60 mRNA的阳性表达量呈先升高后降低的趋势（p<0.01）。在热应激组雏鸡空肠组织中，HSP60蛋白表达水平升高（p<0.01），而HSP60 mRNA表达水平降低（p<0.05）。而热应激组回肠组织中HSP60蛋白表达水平先升高后降低（p<0.05、p<0.01），HSP60 mRNA表达水平则升高（p<0.01）。热应激组雏鸡十二指肠组织中HSP70蛋白表达水平升高（p<0.01），HSP70 mRNA表达水平降低（p<0.05）；而空肠、回肠组织中HSP70蛋白及HSP70 mRNA的表达水平均升高（空肠p<0.01，回肠p<0.05）。热应激组雏鸡十二指肠组织中HSP90蛋白表达水平先降低后升高（p<0.01），HSP90 mRNA表达水平升高（p<0.01）。热应激组空肠组织中HSP90蛋白及HSP90 mRNA的表达水平均升高（p<0.05）。热应激组回肠组织中HSP90蛋白表达水平降低，而HSP90 mRNA表达水平升高（p<0.01）。上述结果表明，热应激可干扰雏鸡小肠组织中HSP60、HSP70及HSP90的表达。