FGFR2 Signaling Underlies p63 Oncogenic Function in Squamous Cell Carcinoma

This study was designed to examine the requirement for the p63 transcription factor in Squamous Cell Carcinoma (SCC) tumor maintenance in an in vivo murine system. A tamoxifen-inducible Keratin 14-driven Cre recombinase transgene was used to conditionally excise p63 in advanced murine SCC tumors. These data show the context-dependent regulation of p63 target genes in cancer. Total RNA from murine Squamous Cell Carcinoma tumors was examined 1-3 days following genomic excision of TP63 in Keratin 14-expressing tumor cells.

本研究旨在探究体内小鼠模型中，鳞状细胞癌（Squamous Cell Carcinoma, SCC）的肿瘤维持过程对p63转录因子的需求。本研究使用他莫昔芬诱导型、角蛋白14（Keratin 14）驱动的Cre重组酶转基因，对晚期小鼠鳞状细胞癌肿瘤中的p63实施条件性敲除。本研究所得数据揭示了癌症中p63靶基因的上下文依赖调控规律。我们对表达角蛋白14的肿瘤细胞内TP63基因组敲除后1-3天的小鼠鳞状细胞癌肿瘤总RNA进行了检测。