Profiling of IL-33 target genes incytotoxic T cells, T helper 1 and T helper 2 cells. Profiling of IL-33 target genes incytotoxic T cells, T helper 1 and T helper 2 cells

To decipher the T cell subset-specific consequences of IL-33 sensing, we profiled early changes in transcriptional activity of in vitro differentiated CTLs, Th1 cells and Th2 cells upon short-term stimulation with IL-33. Overall design: Comparative gene expression profiling analysis of the effects of IL-33 on CTLs, Th1 and Th2 cells using RNA-Seq and single-cell RNA-Seq of WT- and Il1rl1-ExAB-KO CTLs after an acute LCMV infection.

为解析白介素33（IL-33）感知所引发的T细胞亚群特异性生物学效应，我们对经IL-33短期刺激后的体外分化细胞毒性T淋巴细胞（Cytotoxic T Lymphocytes, CTLs）、Th1细胞及Th2细胞的转录活性早期变化开展了基因表达谱分析。实验整体设计：本研究采用RNA测序（RNA-Seq）与单细胞RNA测序（single-cell RNA-Seq）技术，对急性淋巴细胞性脉络丛脑膜炎病毒（Lymphocytic Choriomeningitis Virus, LCMV）感染后的野生型（Wild Type, WT）及Il1rl1-ExAB敲除（Il1rl1-ExAB-KO）细胞毒性T淋巴细胞进行测序，以此对比分析IL-33对CTLs、Th1细胞及Th2细胞的调控效应。