GNPS - PAP-gamma associates with PAXT nuclear exosome to control the stability of PROMPT ncRNAs

Endogenous ZFC3H1 was tagged in 293T cells. Dignam cellular extracts where subjected to sequential FLAG and HA immunoprecipitation and elution. Complexes were then analyzed using mass spectrometry at the taplin mass spectrometry facility. The 2 samples are FLA-HA IP in wild-type 293T cells and Flag-HA IP in FH-ZFC3H1 293T cells.

在293T细胞中对内源ZFC3H1完成了标签标记。经Dignam法提取的细胞裂解液依次进行FLAG与HA免疫沉淀（immunoprecipitation, IP）及洗脱处理。所得复合物随后被送至塔普林质谱分析中心（Taplin Mass Spectrometry Facility）进行质谱（mass spectrometry, MS）分析。本实验设置两个样本：野生型293T细胞的FLA-HA免疫沉淀样本，以及FH-ZFC3H1 293T细胞的Flag-HA免疫沉淀样本。