Single-nucleotide–level mapping of DNA regulatory elements that control fetal hemoglobin expression (histone ChIP-Seq)

gRNA screening for HbF+ and HbF- samples were performed to identify candidate cis-regulatory elements. CRISPR pooled gRNA screening, RNA-seq, ChIP-seq and ATAC-seq were performed

为鉴定候选顺式调控元件（cis-regulatory elements），本研究针对胎儿血红蛋白阳性（HbF+）与胎儿血红蛋白阴性（HbF-）样本完成了向导RNA（guide RNA，gRNA）筛选实验。此外，还开展了CRISPR混合向导RNA筛选、RNA测序（RNA-seq）、染色质免疫共沉淀测序（ChIP-seq）以及转座酶可及性测序（ATAC-seq）实验。