Differential expression data for clonal EL4 mouse lymphoma cell lines

Our group originally developed clonal cell lines by limiting dilution of the parental cell line, and also stably transfected one of these cell lines with human phospholipase D2. The expression analysis was done to examine differences between a non-metastatic cell line (WT2), a metastatic cell line (V&), and a highly metastatic cell line (C5) that had been transfected with hPLD2. The cell lines, which had been serum-starved overnight, were analyzed without further treatment.

本研究团队最初通过对亲本细胞系进行有限稀释法构建了克隆细胞株，并采用人类磷脂酶D2（human phospholipase D2）对其中一株克隆细胞系完成了稳定转染。本研究通过表达分析，对比了非转移细胞系（WT2）、转移细胞系（V&）以及经hPLD2转染的高转移细胞系（C5）之间的表达差异。所有经整夜血清饥饿处理的细胞系均未施加额外干预，直接开展分析检测。