Developmental and housekeeping transcriptional programs in Drosophila require distinct chromatin remodelers (PROseq). Developmental and housekeeping transcriptional programs in Drosophila require distinct chromatin remodelers (PROseq)

Determine the direct effects of chromatin remodeler on transcription using inducible protein degradation combined with nascent transcriptomics. Overall design: Nascent RNA transcription measured by PRO-seq upon AID-mediated depletion of chromatin remodelers. All cell lines have OsTir1 expression from the Act5c locus, and AID tagged gene indicated in genotype. All experiments were performed in 2 biological replicates.

本研究采用诱导型蛋白质降解技术联合新生转录组学（nascent transcriptomics），旨在阐明染色质重塑因子（chromatin remodeler）对转录过程的直接调控效应。整体实验设计：通过PRO-seq检测染色质重塑因子经AID介导的降解后的新生RNA转录水平；所有细胞系均在Act5c基因座上表达OsTir1，且基因型中标注了带有AID标签的靶基因；所有实验均设置2次生物学重复。