Table 1_Staphylococcus epidermidis SAS1: new probiotic candidate for obesity and allergy treatment their mechanistic insights and cytotoxicity evaluation.docx

BackgroundProbiotics are live bacteria that provides numerous healthy and beneficial effects to the consumers. The present study aimed to investigate the effect of a probiotic candidate Staphylococcus epidermidis SAS1, in immunoregulation and obesity management.
Methods: This probiotic candidate was isolated from a soil sample collected from a region of fruit waste decomposition. In vitro cytotoxicity was assessed using the THP-1 (human leukemia monocytic cell line) cells using MTT assay.
ResultsAn IC50 value of 47.52 ± 0.18 μg/mL and cell shrinkage were observed along with the release of cellular content of THP-1 cells. The higher production of reactive oxygen species and lesser release of interleukins (IL-4, 5, and 13) are attributed to the antiallergic potential of this strain. Furthermore, in vitro cytotoxicity evaluation using 3T3-L1 cells identified this strain as a promising candidate for anti-obesity treatment. The observed IC50 value was 514.4 ± 0.061 μg/mL.
DiscussionThis extract was shown to have good lipase-inhibiting enzyme activity and was reported to prevent adipogenesis, depicted by increased HDL levels and decreased LDL and triglyceride levels. These results suggested that Staphylococcus epidermidis SAS1 may have therapeutic use in the treatment of obesity and allergies.

背景：益生菌（Probiotics）是一类对宿主具有多种健康有益作用的活菌体。本研究旨在探讨候选益生菌表皮葡萄球菌（Staphylococcus epidermidis）SAS1在免疫调节与肥胖管理中的作用。
方法：该候选益生菌从果渣分解区域采集的土壤样本中分离得到。采用MTT比色法（MTT assay）对THP-1细胞（人白血病单核细胞系）开展体外细胞毒性评价。
结果：实验测得THP-1细胞的半数抑制浓度（IC50）为47.52±0.18 μg/mL，同时观察到细胞皱缩及细胞内容物释放。该菌株可诱导活性氧（reactive oxygen species）水平升高，同时降低白细胞介素（interleukins，IL-4、IL-5、IL-13）的释放量，这与其抗变态反应潜能密切相关。此外，通过3T3-L1细胞系开展的体外细胞毒性评价显示，该菌株是极具潜力的抗肥胖治疗候选菌株，测得其IC50值为514.4±0.061 μg/mL。
讨论：该菌株提取物表现出良好的脂肪酶抑制活性，且可通过提升高密度脂蛋白（HDL）水平、降低低密度脂蛋白（LDL）与甘油三酯水平来抑制脂肪生成。上述结果表明，表皮葡萄球菌SAS1有望用于肥胖症与变态反应性疾病的临床治疗。