Single-cell RNA sequencing reveals the expansion of circulating tissue-homing B cell subsets in secondary acute dengue viral infection

We subsetted B cells from the integrated Seurat object deposited in Mendeley under URL : https://data.mendeley.com/datasets/6ry3x7r8hf/3. We re-clustered B cells, plasma cells, and plasmablasts using the harmony package with default parameters. The B cell subsets were then annotated using established canonical marker genes described in TableS1.

我们从存储于孟德雷（Mendeley）平台、对应链接为https://data.mendeley.com/datasets/6ry3x7r8hf/3的整合Seurat对象中提取B细胞子集。随后采用Harmony工具包（harmony）的默认参数，对B细胞、浆细胞及浆母细胞进行重新聚类。最后借助表S1中记载的已确立的经典标记基因，完成B细胞子集的注释。