RNA-seq of homogenized lungs of APOE knock-in mice during COVID-19 I. RNA-seq of homogenized lungs of APOE knock-in mice during COVID-19 I

Here we analyze the transcriptional profiles of homogenized lungs resected from APOE2, APOE3, and APOE4 knock-in mice in the absence of infection (day 0) and on days 2 and 4 post infection with SARS-CoV-2 MA10. Weighted gene co-expression analysis identified gene modules enriched for genes implicated in T and B cell activation to be downregulated in APOE2 and APOE4 mice during COVID-19 progression. Overall design: Weighted gene co-expression network analysis and pathway analysis of RNA-sequencing data.

本研究分析了APOE2、APOE3、APOE4敲入小鼠切除的匀浆化肺组织的转录组谱，样本采集涵盖未感染状态（感染后第0天）以及感染严重急性呼吸综合征冠状病毒2（SARS-CoV-2）MA10株后第2天、第4天三个时间点。经加权基因共表达分析（Weighted gene co-expression analysis）发现，在新冠病毒感染疾病进程中，APOE2与APOE4小鼠体内富集于T、B细胞活化相关基因的基因模块呈现下调表达。整体实验设计：针对RNA测序（RNA-sequencing）数据开展加权基因共表达网络分析（Weighted gene co-expression network analysis）与通路分析。