The immunological comparison of control PDA tumors and Krt19-edited PDA tumors. The immunological comparison of control PDA tumors and Krt19-edited PDA tumors

Our studies indicate that cancer cells of carcinomas are coated with CXCL12 chemokine in covalent conjugation with keratin-19 (KRT19). Murine pancreatic ductal adenocarcinomas (PDA) formed with Krt19-edited cancer cells lack the CXCL12-coating and are infiltrated with T cells, compared to tumors formed with control sgScramble PDA cells. To probe the immunological role of this CXCL12-coating thoroughly, bulk RNA sequencing of subcutaneous (s/c) murine sgScramble PDA tumors and Krt19-edited PDA tumors were conducted. Overall design: sgScramble PDA tumors vs sgKrt19 PDA tumors

本研究证实，实体癌的癌细胞表面被与角蛋白19（KRT19）共价结合的CXCL12趋化因子所包被。相较于转染阴性对照sgScramble的胰腺导管腺癌细胞形成的肿瘤，由经Krt19编辑的癌细胞构建的小鼠胰腺导管腺癌（PDA）缺失CXCL12包被，且肿瘤内浸润有T细胞。为全面探究该CXCL12包被的免疫学功能，研究人员对皮下（s/c）生长的sgScramble组小鼠PDA肿瘤与Krt19编辑组小鼠PDA肿瘤开展了批量RNA测序。整体实验设计：sgScramble PDA肿瘤 vs sgKrt19 PDA肿瘤