A compendium of long non-coding RNAs transcriptional fingerprint in multiple myeloma.. A compendium of long non-coding RNAs transcriptional fingerprint in multiple myeloma.

Multiple myeloma (MM) is a malignant proliferation of bone marrow plasma cells (PCs) characterized by highly heterogeneous genetic background and clinical course, and whose pathogenesis remains largely unknown. Long ncRNAs (lncRNAs) are a large class of non-protein-coding RNA, involved in many physiological cellular and genomic processes as well as in carcinogenesis, cancer metastasis and invasion. Although still in its infancy, the knowledge of the role of lncRNAs in MM is progressively expanding. Besides studies on selected candidates, lncRNAs expression at genome-wide transcriptome level is confined to microarray technologies, thus investigating a limited collection of transcripts. Herein, we assessed the lncRNAs expression profiling by RNA-sequencing in a cohort of 30 MM patients, aimed at defining a comprehensive catalogue of lncRNAs specifically associated with the main MM molecular subgroups and genetic alterations. We identified 391 deregulated lncRNAs, 67% of which were also detectable and validated by whole-transcript microarrays. In addition, we identified a list of lncRNAs, with potential relevance in MM, co-expressed and in close proximity to genes that might undergo a cis-regulatory relationship. Herein, we assessed the lncRNAs expression profiling by RNA-sequencing in a cohort of 30 MM patients, aimed at defining a comprehensive catalogue of lncRNAs specifically associated with the main MM molecular subgroups and genetic alterations. We identified 391 deregulated lncRNAs, 67% of which were also detectable and validated by whole-transcript microarrays. In addition, we identified a list of lncRNAs, with potential relevance in MM, co-expressed and in close proximity to genes that might undergo a cis-regulatory relationship. Overall design: Total RNA samples from highly purified plasma cells of 30 MM cases at onset and 4 normal controls (purchased from Voden, Medical Instruments IT).

多发性骨髓瘤（Multiple myeloma, MM）是一类以高度异质性的遗传背景与临床病程为特征的骨髓浆细胞（plasma cells, PCs）恶性增殖性疾病，其发病机制在很大程度上尚未阐明。长链非编码RNA（long non-coding RNAs, lncRNAs）为一大类非蛋白编码RNA，参与诸多细胞生理活动、基因组调控过程以及癌变、肿瘤转移与侵袭过程。尽管目前针对lncRNAs在MM中作用的研究尚处于起步阶段，但相关认知正逐步深化。除针对单一候选靶点的研究外，此前全基因组转录组层面的lncRNAs表达分析仅能依托微阵列技术进行，因此仅可对有限的转录本集合开展探究。
在此，本研究通过RNA测序（RNA-sequencing）对30例MM患者队列的lncRNAs表达谱进行分析，旨在构建与MM主要分子亚型及遗传改变特异性相关的lncRNAs综合目录。本研究共鉴定出391个表达失调的lncRNAs，其中67%可通过全转录组微阵列技术检测并验证。此外，本研究还鉴定出一批在MM中具有潜在相关性的lncRNAs，这些lncRNAs与可能存在顺式调控关系的基因共表达且位置邻近。
在此，本研究通过RNA测序（RNA-sequencing）对30例MM患者队列的lncRNAs表达谱进行分析，旨在构建与MM主要分子亚型及遗传改变特异性相关的lncRNAs综合目录。本研究共鉴定出391个表达失调的lncRNAs，其中67%可通过全转录组微阵列技术检测并验证。此外，本研究还鉴定出一批在MM中具有潜在相关性的lncRNAs，这些lncRNAs与可能存在顺式调控关系的基因共表达且位置邻近。
整体实验设计：采集30例初诊MM患者的高纯度浆细胞以及4例正常对照的总RNA样本，正常对照样本购自Voden医疗器械公司（Voden, Medical Instruments IT）。