Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [RNA-Seq]

We report single-cell gene expression profiling of the zebrafish melanoma cell lines ZMEL1-PRO and ZMEL1-INV under multiple in vitro and in vivo (zebrafish transplant) conditions Overall design: Single-cell RNA-seq of ZMEL1-PRO and ZMEL1-INV cells in one of four conditions: 1) in vitro individual culture; 2) in vitro co-culture; 3) in vivo primary tumors; and 4) in vivo metastases

本研究报道了斑马鱼黑色素瘤细胞系ZMEL1-PRO与ZMEL1-INV在多种体外（in vitro）及体内（in vivo，斑马鱼移植）条件下的单细胞基因表达谱（single-cell gene expression profiling）。实验设计概述：对ZMEL1-PRO和ZMEL1-INV细胞进行单细胞RNA测序（single-cell RNA-seq），样本共分为四种处理条件：1）体外单独培养；2）体外共培养；3）体内原发肿瘤；4）体内转移灶