Cast iron drinking water pipe biofilm community analysis

Microbial biofiolm samples were collected from a section of a full-scale >100-year-old chloraminated cast iron drinking water main. Illumina MiSeq was used to sequence the V4 hypervariable region of 16S rRNA genes in bacterial and archaeal communities. Blank/no template control and positive/mock community control samples were included (n = 26).研究人员从一段实际运行的、服役超百年的加氯胺铸铁饮用水干管区段采集了微生物生物膜样本。采用Illumina MiSeq测序仪对细菌及古菌群落的16S rRNA基因V4高变区进行测序。本次研究共纳入26份样本，其中包含空白（无模板）对照与阳性（模拟群落）对照样本。