Wild-type Zebrafish subjected to swim-training

Genome-wide microarray analysis of the effects of swim-training on zebrafish larval development. Zebrafish were subjected to swim-training from 5 days post fertilization (dpf) until 10 dpf. Subsequently, we performed a genome-wide microarray analysis of trained and control fish at 10 dpf. The goal of the project was to investigate the effects of swim-training on the gene expression level during zebrafish larval development Two-condition experiment: control vs trained fish. RNA was isolated from 10 control fish and from 10 trained fish. Subsequently, each sample was labeled with Cy3 and Cy5 in order to correct for dye bias. Control-Cy3 and Trained-Cy5 were hybridized on array 1 and Trained-Cy5 and Control-Cy3 were hybridized on array 2.

本研究针对游泳训练对斑马鱼幼体发育的影响开展全基因组微阵列分析。实验所用斑马鱼于受精后5天（days post fertilization, dpf）开始接受游泳训练，直至受精后10天（dpf）。随后，我们在受精后10天（dpf）对训练组与对照组斑马鱼开展全基因组微阵列分析。本项目旨在探究游泳训练对斑马鱼幼体发育过程中基因表达水平的影响。本实验为双组对照设计：对照组与训练组斑马鱼。从10尾对照组斑马鱼与10尾训练组斑马鱼中分离RNA。为校正染料偏差，每份样本分别以Cy3与Cy5进行标记。将标记为Control-Cy3与Trained-Cy5的样本在芯片1上进行杂交，将标记为Trained-Cy5与Control-Cy3的样本在芯片2上进行杂交。