Indole-3-Carbinol Preferentially Target ERα-Positive Breast Cancer Cells.. Homo sapiens

Indole-3-carbinol (I3C) is a natural anti-carcinogenic compound found at high concentrations in Brassica vegetables. ER-positive cell lines demonstrated the greatest sensitivity to the anti-tumor effects of I3C compared to ER-negative breast cancer cell lines. Gene expression analysis was performed to identify genes and pathways that accounted for sensitivity to I3C. Microarray analysis performed using Illumina HT-12 v4 expression arrays Overall design: A total of 36 samples were analyzed with six breast cancer cell lines treated with either the vehicle control or the drug Indole-3-carbinol in triplicate. The cell lines were: MCF-7, T47D, ZR751(sensitive to the drug, apoptosis/growth arrest) and MDA-MB-231, MDA-MB-157, and MDA-MB-436 (insensitive to the drug). Sensitive cell lines are of the luminal subtype and insensitive cell lines are of the basal subtype.

吲哚-3-甲醇（Indole-3-carbinol，I3C）是一种在十字花科蔬菜中高浓度存在的天然抗癌化合物。相较于雌激素受体阴性（ER-negative）乳腺癌细胞系，雌激素受体阳性（ER-positive）细胞系对I3C的抗肿瘤效应展现出最强的敏感性。本研究开展基因表达分析，以鉴定与I3C敏感性相关的基因及信号通路，并采用Illumina HT-12 v4表达芯片进行微阵列分析。实验整体设计：共分析36份样本，6种乳腺癌细胞系分别经溶剂对照或吲哚-3-甲醇处理，每组设置3次生物学重复。所用细胞系包括：对药物敏感（可诱导细胞凋亡/生长阻滞）的MCF-7、T47D、ZR751，以及对药物不敏感的MDA-MB-231、MDA-MB-157和MDA-MB-436。其中敏感细胞系属于管腔型亚型，不敏感细胞系则属于基底型亚型。