Implications of saline water irrigation and soil straw amendment for the microbial diversity in semiarid soil and wheat rhizosphere

The effects of salinization and straw amendment were studied on the microbial community in a soil from a semiarid scrubland. Soil with or without straw amendment was irrigated with non-saline water or water with low or high salinity in a greenhouse experiment. After this salinization treatment, wheat seedlings were planted into the soil and grown for seven weeks. The microbial communities in the bare soil before planting and in the wheat rhizosphere were studied by Illumina MiSeq sequencing of 16S rRNA gene amplicons.

本研究以半干旱灌丛地土壤为研究对象，探究盐渍化与秸秆还田处理对其微生物群落的影响。本温室试验设置两类土壤预处理：施加秸秆与不施加秸秆，分别采用非盐渍水、低盐度水及高盐度水进行灌溉。待盐渍化处理完成后，向土壤中移栽小麦幼苗并培养7周。本研究通过对16S rRNA基因扩增子（16S rRNA gene amplicons）开展Illumina MiSeq测序（Illumina MiSeq），分析了种植前裸土及小麦根际土壤的微生物群落组成。