Raw Data and RCode: Heatwave-like events affect drone production and brood-care behaviour in bumblebees

Raw data, research codes, and table (statistical models) for manuscript "Heatwave-like events affect drone production and brood-care behaviour in bumblebees".

Objective of study: Investigate the impact of simulated heatwaves on the performance of queenless microcolonies of Bombus terrestris audax under laboratory conditions.

Mortality (File: Mortality_status.csv):
The mortality of workers was measured over the five-week period on a daily basis (number of dead workers per microcolony) (See Manuscript Codes - Colony Performance.R and/or Table_Models.docx for model).

Colony Growth (File: Colony_Growth.csv):
The number of drones per microcolony and treatment was measured at the end of the five-week period (See Manuscript Codes - Colony Performance.R for model). The exact number of drones produced per microcolony is shown in File: Colony_Growth.csv.
The number of discarded larvae per microcolony was measured on a daily basis throughout the five-week period (See Manuscript Codes - Colony Performance.R for model). The number of eggs, larvae, and pupae per microcolony is shown in File: Colony_Growth.csv.

Food Consumption (File: Food_consumption.csv):
The volume of syrup feeders was taken every three days over the five-week period and the total average was obtained (nine measurements per microcolony); ml) (See Manuscript Codes - Colony Performance.R for model).

Weight change (File: Weight_data.csv):
Weight change of workers was obtained by collecting each individual bee with a marking cage (with the weight of the cage subtracted) and subsequently weighing them individually once a week over a five-week period using an analytical balance (body mass; mg) (See Manuscript Codes - Colony Performance.R for model).

Body Size and Body Mass (File: Drone_mass_size.csv):
Body size (intertegular distance; mm) was obtained by measuring the distance between the bases of the wings on the bee thorax with a digital calliper after the five-week period, and the dry body mass of drones (dried at 60 ºC for five consecutive days after the five-week period and weighed to the nearest mg (VWR® LA Classic Analytical Balance)) (See Manuscript Codes - Colony Performance.R for models).

Behaviour (File: Behaviour_Data.csv):
The total number of workers engaged in wing fanning (fanning with spread wings for at least 5 s) and the total number of workers incubating the brood (positioned on top of brood with curled abdomen for at least 5 s) were recorded throughout the heatwave simulation period. The behaviour (h-i) of workers in each microcolony was observed continuously for 7 minutes divided into 5 intervals (1 min 40 s each) on a daily basis during the heatwave simulation for a total of five days (total of 35 min per microcolony) (See Manuscript Codes - Colony Performance.R for models).

Author Names and Affiliations: Yanet Sepúlveda, Elizabeth Nicholls, Wiebke Schuett, Dave Goulson. School of Life Sciences, University of Sussex, Falmer, Brighton, BN1 9QG, UK.

本数据集配套论文《类热浪事件影响熊蜂雄蜂生产与育幼行为》的原始数据、研究代码及统计模型表格。
研究目标：在实验室环境中探究模拟热浪对明亮熊蜂（Bombus terrestris audax）无蜂王微型蜂群表现的影响。

死亡率数据（文件：Mortality_status.csv）：在为期5周的实验周期内每日监测工蜂死亡率（每微型蜂群的死工蜂数量），相关模型详见论文代码Colony Performance.R或Table_Models.docx。

蜂群生长数据（文件：Colony_Growth.csv）：在5周实验周期结束时统计每个微型蜂群各处理组的雄蜂数量，相关模型详见论文代码Colony Performance.R。每个微型蜂群的实际雄蜂生产数量已记录于Colony_Growth.csv文件中。实验期间每日统计每个微型蜂群的丢弃幼虫数量，相关模型详见论文代码Colony Performance.R。每个微型蜂群的卵、幼虫及蛹的数量已记录于Colony_Growth.csv文件中。

食物消耗量数据（文件：Food_consumption.csv）：在5周实验周期内每3天测定一次糖浆饲喂器的剩余体积，最终计算总平均值（每个微型蜂群共9次测定值，单位：ml），相关模型详见论文代码Colony Performance.R。

体重变化数据（文件：Weight_data.csv）：通过标记笼抓取每只工蜂（需扣除标记笼自身重量），在5周周期内每周使用分析天平单独称量一次工蜂体重（体质量，单位：mg），相关模型详见论文代码Colony Performance.R。

体型与体质量数据（文件：Drone_mass_size.csv）：实验周期结束后，使用数字游标卡尺测量熊蜂胸部翅基间距（单位：mm）以获取工蜂体型数据；同时将雄蜂置于60℃环境下连续烘干5天，使用VWR® LA Classic 分析天平称取其干体质量（精确至1mg），相关模型详见论文代码Colony Performance.R。

行为学数据（文件：Behaviour_Data.csv）：在热浪模拟期间持续记录工蜂扇翅行为（展开翅膀持续扇动至少5秒）的总次数，以及育幼行为（腹部卷曲并停留于蜂团上方至少5秒）的工蜂总数量。热浪模拟期间每日对每个微型蜂群的工蜂行为进行连续7分钟的观察，分为5个间隔时段（每个时段1分40秒），共持续5天（每个微型蜂群总观察时长为35分钟），相关模型详见论文代码Colony Performance.R。

作者及单位：Yanet Sepúlveda, Elizabeth Nicholls, Wiebke Schuett, Dave Goulson。英国萨塞克斯大学生命科学学院，法尔默，布莱顿，BN1 9QG，英国。