Expression yields and localization of recombinant MBP in various hosts.

Western blots (Fig 3) were subjected to densitometric analysis using ImageJ software [26]. Results were quantified using calibration standards on each Western blot. From these data relative distributions of MBP between the extracellular and intracellular compartments were calculated. “nd” means “not detected”. Expression patterns of MBP using pONE vectors demonstrate that proteins of interest are expressed and directed to the relevant compartment.

采用ImageJ软件[26]对免疫印迹（Western blot，图3）进行光密度分析。每次免疫印迹实验均通过校准标准品对检测结果进行定量。基于上述实验数据，可计算得到髓鞘碱性蛋白（MBP）在细胞外与细胞内区室中的相对分布。“nd”代表“未检测到”。采用pONE载体得到的MBP表达模式实验结果证实，目的蛋白可成功表达并靶向递送至相应的细胞区室。