A sensitive biomimetic enzyme-linked immunoassay method based on Au@Pt@Au composite nanozyme label and molecularly imprinted biomimetic antibody for histamine detection

In this paper, a sensitive biomimetic enzyme-linked immunoassay (BELISA) method based on an Au@Pt@Au composite nanozyme label was developed for histamine (HA) detection. A molecular imprinted polymer prepared directly on the 96-well plate served as a biomimetic antibody. A BELISA method was developed for HA standard solutions at concentrations ranging from 0.01 to 100.00 mg/L. Under optimal conditions, the novel BELISA method had a low limit of detection (0.069 mg/L) and high sensitivity (7.20 mg/L). The HA spiked in yellow rice wine and liqueur samples were detected by this BELISA method with the recoveries ranging from 84.28% to 108.82%. There was no significant difference of the results between the BELISA method and HPLC for the detection of HA in the red and white grape wine samples.

本文开发了一种基于Au@Pt@Au复合纳米酶标记物的敏感型仿生酶联免疫吸附测定法（BELISA），用于组胺（HA）的检测。直接在96孔板上制备的分子印迹聚合物可作为仿生抗体。本文针对浓度范围为0.01~100.00 mg/L的组胺标准溶液建立了该BELISA检测方法。在最优实验条件下，该新型BELISA方法具备极低的检出限（0.069 mg/L）与较高的灵敏度（7.20 mg/L）。采用该BELISA方法对黄酒与利口酒样品中的加标组胺进行检测，所得回收率介于84.28%~108.82%之间。针对红葡萄酒与白葡萄酒样品中的组胺检测，该BELISA方法的检测结果与高效液相色谱法（HPLC）无显著差异。