Gene expression profiles of MDA-MB-231 breast cancer cell line treated with DMSO, 10 µM DAC, 1 µM DEX, or DAC+DEX for 6 days.

We observed gene expression difference between different groups after MDA-MB-231 treated with DMSO, 10 µM DAC, 1 µM DEX, or DAC+DEX. Data obtained from high-throughput sequencing (Illumina NovaSeq 6000 platform) were transformed into raw sequenced reads by CASAVA base calling and stored in FASTQ format. Gene expression of each groups are listed in raw data files. Some different expression genes between two groups are further validated with qRT-PCR. Overall design: Examination of gene expression difference of MDA-MB-231 cells treated with DMSO, 10 µM DAC, 1 µM DEX, or DAC+DEX for 6 days

我们观察到，经二甲基亚砜（DMSO）、10 μM 地西他滨（DAC）、1 μM 地塞米松（DEX）或DAC与DEX联合处理MDA-MB-231细胞后，不同实验组间存在基因表达差异。
本研究通过Illumina NovaSeq 6000平台开展高通量测序所获数据，经CASAVA碱基识别转换为原始测序读段（reads），并以FASTQ格式存储。
各组的基因表达信息均列于原始数据文件内。
部分两两组间的差异表达基因进一步通过实时定量聚合酶链式反应（qRT-PCR）完成验证。
实验整体设计：对经DMSO、10 μM DAC、1 μM DEX或DAC+DEX联合处理6天的MDA-MB-231细胞的基因表达差异进行检测。