Young and elderly fibroblast cells

Age-associated accumulation of somatic mutations in mitochondrial DNA (mtDNA) has been proposed to be responsible for the age-associated mitochondrial respiration defects found in elderly human subjects. In contrasts, our previous studies proposed that the age-associated respiration defects found in human fibroblasts are caused not by mtDNA mutations. To addressed these controversial issues, we carried out microarray analysis of two young (TIG3S and TIG121) and two elderly (TIG107 and TIG102) fibroblasts. Young (TIG3S and TIG121) and elderly (TIG107 and TIG102) fibroblasts are analyzed.

线粒体DNA（mitochondrial DNA, mtDNA）的体细胞突变随年龄积累的现象，曾被提出为老年人类受试者所出现的年龄相关性线粒体呼吸功能缺陷的致病诱因。与之相反，我们此前的研究提出，人类成纤维细胞中观察到的年龄相关性呼吸功能缺陷并非由线粒体DNA突变所导致。为解决这一存在争议的学术问题，我们对两株年轻成纤维细胞（TIG3S与TIG121）以及两株老年成纤维细胞（TIG107与TIG102）开展了微阵列分析。本次研究的分析对象即为上述年轻及老年成纤维细胞株。