Implementation of Genomic Variant Calling Using GATK4, SPARK, WDL, CROMWELL and DOCKER Over Simulated Ebola NGS Dataset.

Ebola genome is manually mutated to contain structural variants. There are seven mutated ebola genomes each one for large deletion, insertion, duplication, translocation, inversion, complex variant1 (consecutive three mutations - insertion, duplication and deletion) and complex variants2 (consecutive three mutations - deletion, duplication and deletion). All insertions are novel sequence insertion. Paired-end illumine RNASEQ reads are simulated in fastq format.本数据集通过人工诱变手段使埃博拉基因组携带各类结构变异（structural variants），共包含7条诱变后的埃博拉基因组，分别对应大片段缺失、插入、重复、易位、倒位、复合变异1（连续三种突变：插入、重复与缺失）以及复合变异2（连续三种突变：缺失、重复与缺失）。所有插入均为新型序列插入。本数据集以FASTQ格式模拟生成双端Illumina RNA测序（RNASEQ）读段。