Symphony pre-built single-cell reference atlases

Pre-built Symphony reference objects that can be downloaded and used to map new query datasets. The Symphony algorithm is used to perform reference mapping to these atlases. Preprint: https://www.biorxiv.org/content/10.1101/2020.11.18.389189v2 Usage: https://github.com/immunogenomics/symphony <strong>References available for download:</strong> 10x PBMCs Atlas (pbmcs_10x_reference.rds) Pancreatic Islet Cells Atlas (pancreas_plate-based_reference.rds) Fetal Liver Hematopoiesis Atlas (fetal_liver_reference_3p.rds) Healthy Fetal Kidney Atlas (kidney_healthy_fetal_reference.rds) T cell CITE-seq atlas (tbru_ref.rds) Cross-tissue Fibroblast Atlas (see here) Cross-tissue Inflammatory Immune Atlas (here) Tabula Muris Senis (FACS) Atlas (TMS_facs_reference.rds) To read in a reference into R, one may simply execute: reference = readRDS('path/to/reference_name.rds') Note: To be able to map query datasets into the reference UMAP coordinates, you must also download the corresponding 'uwot_model' file and set the reference$save_uwot_path.可下载并用于映射新查询数据集的预构建Symphony参考对象集。Symphony算法可用于对这些参考图谱执行参考映射操作。 预印本：https://www.biorxiv.org/content/10.1101/2020.11.18.389189v2 使用方法：https://github.com/immunogenomics/symphony 可供下载的参考数据集如下： 10x 外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMC）参考图谱（pbmcs_10x_reference.rds） 胰岛细胞参考图谱（pancreas_plate-based_reference.rds） 胎儿肝脏造血参考图谱（fetal_liver_reference_3p.rds） 健康胎儿肾脏参考图谱（kidney_healthy_fetal_reference.rds） T细胞CITE-seq参考图谱（tbru_ref.rds） 跨组织成纤维细胞参考图谱（详见此处） 跨组织炎症免疫参考图谱（详见此处） 老年小鼠细胞图谱（Tabula Muris Senis，FACS分选）参考图谱（TMS_facs_reference.rds） 若需在R语言中导入参考数据集，仅需执行如下代码：reference = readRDS('path/to/reference_name.rds') 注意：若需将查询数据集映射至统一流形近似与投影（UMAP）参考坐标，还需下载对应的'uwot_model'文件，并设置reference$save_uwot_path参数。