Ska3-microtubule interactions - Ska3 Ensures Timely Mitotic Progression by Interacting Directly With Microtubules and Ska1 Microtubule Binding Domain

The Ndc 80 and Ska complex are the major microtubule-binding factors of the kinetochore responsible for maintaining chromosome-microtubule coupling during chromosome segregation. We previously showed that the Ska1 subunit of the Skacomlex binds dynamic microtubules. This project demonstrated that Ska3 subunit is required to modulate the microtubule binding capability of the Ska complex by directly interacting with tubulin monomer and indirectly by interacting with tubulin contacting regions of Ska1.

Ndc 80与Ska复合物（Ska complex）是动粒（kinetochore）的主要微管结合因子，在染色体分离过程中负责维持染色体与微管间的偶联。我们既往的研究表明，Ska复合物的Ska1亚基可结合动态微管。本研究证实，Ska3亚基可通过两种途径调控Ska复合物的微管结合能力：一是直接与微管蛋白单体相互作用，二是间接通过结合Ska1的微管结合区域实现调控。