Effects of Poly (ADP-ribose) Polymerase Inhibitor Treatment on the Repair Process of Ischemic Acute Kidney Injury. Effects of Poly (ADP-ribose) Polymerase Inhibitor Treatment on the Repair Process of Ischemic Acute Kidney Injury

The role of PARP inhibitors in the repair process of renal remains unclear. The effects of JPI-289, a novel PARP inhibitor, during the healing phase after renal ischemia-reperfusion injury were investigated. Overall design: scRNAseq were perfomed with post-ischemic kidneys. JPI-289 100 mg/kg (n = 1) or saline (n = 1) was injected into the peritoneal cavity immediately before reperfusion during bilateral renal ischemia-reperfusion surgery. Mice were sacrificed at 24 h following bilateral ischemia-reperfusion surgery

聚腺苷二磷酸核糖聚合酶（PARP）抑制剂在肾脏修复过程中的作用目前仍不明确。本研究探讨了新型PARP抑制剂JPI-289在肾脏缺血再灌注损伤后愈合阶段的作用效果。
实验整体设计：对缺血后肾脏组织进行单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）。在双侧肾脏缺血再灌注手术中，于再灌注即刻向小鼠腹腔内注射100 mg/kg的JPI-289（n=1）或生理盐水（n=1）；并于双侧肾脏缺血再灌注手术后24小时处死小鼠。