RNA profiling of four DLD1 colorectal cancer cell derivatives that recapitulate EMT/MET transitions during metastasis.. Homo sapiens

RNA profiling of a cellular model of colorectal cancer progression, which is based on four defined derivates of a CRC cell line that resulted from EMT/MET transitions and phenotypically recapitulate the metastatic cascade: epithelial-like primary tumor, mesenchymal-like tumor cells growing in soft agar or as colonospheres, and mouse xenografts of the latter resulting in lung-metastasis comprised of epithelial-state cells. Overall design: Colorectal cancer cell line DLD1 and its derivatives were maintained in McCoy`s 5A Medium (Invitrogen) containing 10% fetal bovine serum (FBS, Invitrogen), 100 units/ml penicillin and 0.1 mg/ml streptomycin.

结直肠癌进展细胞模型的RNA谱分析（RNA profiling）：该模型基于一株结直肠癌（Colorectal Cancer, CRC）细胞系的四种经明确鉴定的衍生株，这些衍生株由上皮间质转化（Epithelial-Mesenchymal Transition, EMT）与间质上皮转化（Mesenchymal-Epithelial Transition, MET）过程产生，且在表型层面重现了肿瘤转移级联反应：包括上皮样原发肿瘤细胞、可在软琼脂中生长或形成结肠球的间质样肿瘤细胞，以及将上述间质样肿瘤细胞接种至小鼠体内构建的异种移植模型所形成的、由上皮状态细胞构成的肺转移瘤。总体实验设计：结直肠癌细胞系DLD1及其衍生株均培养于McCoy's 5A培养基（Invitrogen）中，该培养基添加有10%胎牛血清（Fetal Bovine Serum, FBS，Invitrogen）、100单位/ml青霉素及0.1 mg/ml链霉素。