Genome-wide analysis of gene expression in lung cells from metformin treated Mycobacterium tuberculosis infected mice. Mus musculus

Analysis of Metfromin induced changes in the lung cells of Mycobacterium tuberculosis infected mouse at gene expression level. The hypothesis tested in the present study was whether metformin has any effect on the host immune response in Mycobacterium tuberculosis infected mice? Results provide important information on the effect of metformin on the inflammatory response and immune activation associated with mycobacterial infection. In conclusion, Metfromin normalizes the chronic inflammation associated with Mycobacterium tuberculosis infection. Overall design: Total RNA obtained from lung cells of untreated and metformin (500 mg/Kg) treated Mycobacterium tuberculosis infected mouse. The treatment was started day 7 post-infection and was given for 30 days post-initiation.本研究针对感染结核分枝杆菌（Mycobacterium tuberculosis）小鼠的肺细胞，在基因表达水平上分析二甲双胍（Metformin）诱导的肺细胞基因表达变化。本研究验证的科学假说为：二甲双胍是否会对感染结核分枝杆菌小鼠的宿主免疫应答产生影响？本研究结果为阐明二甲双胍对分枝杆菌感染相关炎症应答与免疫激活的作用提供了重要参考。综上，二甲双胍可使结核分枝杆菌感染相关的慢性炎症恢复至正常水平。实验设计概况：从未处理组及经二甲双胍（500 mg/kg）处理的感染结核分枝杆菌小鼠的肺细胞中提取总RNA。给药于感染后第7天启动，持续给药30天。