Wirasinha.CDD.2019.txt.zip

The file contains mouse T cell receptor (TCR) sequences collected by multiplex PCR amplification of cDNA molecules followed by Illumina sequencing. Sequences were aligned to the mouse genome using MIGEC software (see doi: 10.1038/nmeth.2960 for details). Except for the header row, each row contains information about a unique TCR nucleotide sequence. Columns 1-11 contain output from MIGEC software. Columns 12-16 contain information about sample origin, detailed as follows: column 12 "sample_id" is an identifier for the sample of origin; column 13 "chain" specifies the TCR chain ("a" = alpha, "b" = beta); column 14 "genotype" specifies the transgene status of donor mouse ("wt" or "bcl2" to indicate the absence or presence of BCL2-tg expression, respectively); column 15 "tcr_b" specifies the TCR beta chain status of donor mouse ("poly" = C57BL/6 inbred mouse strain); column 16 "mouse" is an identifier of the donor mouse; column 17 "organ" specifies organ of origin (thymus, "gut" = small intestine and spleen); column 18 "subset" specifies the T cell subset of origin ("t_p" = pre-selection thymocytes; "t_1" = Type A IELp; "t_1b" = Type B IELp; "t_r" = thymic T-reg; "t_4" = CD4+ T-conv thymocytes; "t_8" = CD8+ T-conv thymocytes; "g8a" = small intestinal CD8aa+ intraepithelial lymphocytes; "s_r" = splenic T-reg; "s_4" = splenic CD4+ naive T cells and "s_8" = splenic CD8+ naive T cells); column 19 is identical to column 18 except that column 19 shows whether the T-reg sample of origin was a CD25+ ("t_u" and "s_u") or CD25– ("t_d" and "s_d") fraction of a thymic or splenic T-reg population.

该文件包含通过多重PCR扩增cDNA分子后经Illumina测序获得的小鼠T细胞受体（T cell receptor, TCR）序列。序列通过MIGEC软件比对至小鼠基因组（详细信息参见doi: 10.1038/nmeth.2960）。除表头行外，每一行对应一条唯一的TCR核苷酸序列。第1至11列为MIGEC软件的输出结果。第12至16列为样本来源信息，具体说明如下：第12列"sample_id"为样本来源标识符；第13列"chain"用于指定TCR链型（"a"代表α链，"b"代表β链）；第14列"genotype"用于标注供体小鼠的转基因状态（"wt"表示无BCL2转基因表达，"bcl2"表示存在BCL2转基因表达）；第15列"tcr_b"用于指定供体小鼠的TCRβ链状态（"poly"代表C57BL/6近交系小鼠）；第16列"mouse"为供体小鼠标识符；第17列"organ"用于标注样本来源器官（胸腺、"gut"代表小肠与脾脏）；第18列"subset"用于标注来源T细胞亚群，具体包括："t_p"为选择前胸腺细胞；"t_1"为A型肠上皮内淋巴细胞前体（IELp）；"t_1b"为B型IELp；"t_r"为胸腺调节性T细胞（T-reg）；"t_4"为CD4+常规胸腺T细胞；"t_8"为CD8+常规胸腺T细胞；"g8a"为小肠CD8aa+肠上皮内淋巴细胞；"s_r"为脾脏调节性T细胞；"s_4"为脾脏CD4+初始T细胞；"s_8"为脾脏CD8+初始T细胞；第19列与第18列内容一致，仅用于区分来源调节性T细胞（T-reg）样本为CD25+（"t_u"和"s_u"）还是CD25–（"t_d"和"s_d"）的胸腺或脾脏T-reg亚群组分。