SH1405686.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的方法，用于对基于DNA的物种假说（DNA-based Species Hypotheses，简称SH）进行界定、识别、交流及相关研究处理。研究人员通过应用一套动态阈值距离区间（0.5%~3.0%），将国际核苷酸序列数据库中的全部真菌ITS（Internal Transcribed Spacer，内部转录间隔区）序列聚类至近似物种级别。所有SH均被赋予以DOI（Digital Object Identifier，数字对象标识符）形式存在的唯一且稳定的标识名称，其分类学与生态学注释信息将通过分布式网络第三方注释工作完成验证。系统会结合SH内所有序列的鉴定结果，尽可能将每个SH与对应的分类单元名称及其所属分类层级（如门、纲、目等）进行关联匹配。研究人员会选取一条经自动或人工指定的序列作为每个SH的代表序列，此类代表序列已发布于（https://unite.ut.ee/repository.php），可供科研群体用于本地序列相似性检索、高通量测序分析流程等科研场景。随着公共真菌ITS序列库规模的持续增长，该系统及其配套数据将自动完成更新迭代。