<b>Effects of miR-29c on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stromal cells</b>

microRNA （miRNA） 是非编码短 RNA 的一个亚类，它指导细胞命运决策，这对细胞增殖和细胞谱系决策很重要。脂肪形成分化极大地促进了白色脂肪组织的发育，涉及miRNA的高度组织化调节。在本研究中，我们筛选并鉴定了 78 种不同表达的猪 BMSC miRNA 在脂肪生成分化过程中。其中，miR-29c在调节增殖和脂肪生成分化中的作用得到了证明和详细说明。具体来说，过表达 miR-29c 抑制 BMSC 的增殖和脂肪生成分化，这在 miR-29c 抑制剂上被逆转。IGF1的干扰抑制了BMSCs的增殖和脂肪形成分化。从机制上讲，miR-29c分别通过靶向IGF1和进一步调节MAPK通路和PI3K-AKT-mTOR通路来调节BMSCs的增殖和脂肪形成分化。总之，我们强调了miR-29c在调节BMSCs增殖和脂肪形成分化方面的重要作用。microRNA (miRNA) is a subclass of non-coding short RNAs that govern cell fate decisions, which are essential for both cell proliferation and cell lineage determination. Adipogenic differentiation greatly promotes the development of white adipose tissue and involves highly organized regulation mediated by miRNAs. In this study, we screened and identified 78 differentially expressed porcine bone marrow mesenchymal stem cell (BMSC) miRNAs during adipogenic differentiation. Among these, the role of miR-29c in regulating proliferation and adipogenic differentiation was validated and elaborated in detail. Specifically, overexpression of miR-29c suppressed the proliferation and adipogenic differentiation of BMSCs, and this inhibitory effect was reversed by miR-29c inhibitors. Knockdown of IGF1 inhibited the proliferation and adipogenic differentiation of BMSCs. Mechanistically, miR-29c modulates BMSC proliferation and adipogenic differentiation by targeting IGF1, and further regulating the MAPK pathway and PI3K-AKT-mTOR pathway, respectively. Collectively, we highlight the critical role of miR-29c in regulating BMSC proliferation and adipogenic differentiation.