A mannose-sensing AraC-type transcriptional activator regulates intestinal colonization of Vibrio cholerae

In this study, we show that ManR (VC1825) activates the transcription of the mannose operon in M9 medium supplemented with fructose or mannose, and when manR is impaired Vibrio cholerae cells fail to colonize the intestine and host survival is significantly prolonged in a fruit fly Drosophila melanogaster infection model. To identify other target genes of ManR, transcriptome analysis was performed in wild-type V. cholerae and manR mutant strains, , grown on M9 medium supplemented with glucose, fructose, or mannose. Overall design: Transcriptome profiles of WT V. cholerae and manR mutant cells grown on a single carbohydrate as a sole carbon source (glucose, fructose, mannose)

本研究证实，ManR（VC1825）可在添加果糖或甘露糖的M9培养基（M9 medium）中激活霍乱弧菌（Vibrio cholerae）甘露糖操纵子的转录；当manR基因功能受损时，霍乱弧菌无法定植宿主肠道，且在黑腹果蝇（Drosophila melanogaster）感染模型中可显著延长宿主存活时间。为鉴定ManR的其他靶基因，本研究在以葡萄糖、果糖或甘露糖为唯一碳源的M9培养基中培养野生型（wild-type）霍乱弧菌与manR突变菌株，并对其开展转录组分析。实验设计概述：以单一碳水化合物作为唯一碳源（葡萄糖、果糖、甘露糖）培养的野生型霍乱弧菌及manR突变菌株的转录组谱。