Budding index of agar cultures of <i>S.cerevisiae</i>, 210 min after inoculation – in control and after 120 min induction with various inductors (see fig. 4D for the whole budding curve on this medium).

The experiment scheme is given in fig. 3A. Inductor – S.cerevisiae cultures of various age, in YPD (suspension) and on YPD-agar. Recipient – S.cerevisiae culture 15 min after inoculation. Medium content: CH3COONa 0,1%+KH2PO4 0,1%+MgSO4 0,05%+CaCl2 0,01%+NaCl 0,01%+agar 3%, pH5,5.实验方案详见图3A。诱导组：不同菌龄的酿酒酵母（Saccharomyces cerevisiae）培养物，分别采用YPD液体悬浮培养及YPD琼脂平板培养形式。受体组：接种15分钟后的酿酒酵母培养物。 培养基成分：乙酸钠（CH3COONa）0.1% + 磷酸二氢钾（KH2PO4）0.1% + 硫酸镁（MgSO4）0.05% + 氯化钙（CaCl2）0.01% + 氯化钠（NaCl）0.01% + 琼脂3%，pH值5.5。