Comparison between mouse ES/iPS derived neurosphere and mouse primary culture of neurospheres obtained from fetal mouse ganglionic eminence

Recent reports have emphasized the pitfalls of iPSC technology including the potential for immunogenicity of transplanted cells. It is serious safety-related concern for iPSC-based cell therapy. However, preclinical data supporting the safety and efficacy of iPSCs are also accumulating. To address the concern of immunogenicity of ESCs/iPSCs or ESCs/iPSCs-derived neurospheres, global gene expression profiles were compared between undifferentiated mouse ESCs (EB3 line), mouse iPSCs (38C2 line), and ESC/iPSC-derived neurosphere and mouse primary culture of neurosphere obtained from fetal mouse ganglionic eminence. Mouse adult sklin fibroblast was used as a control. We used affymetrix microarrays to compare the global gene expression of neurospheres prepared several origins. Keywords: Expression profiling by array RNA extracted from neurospheres was hybridized to Affymetrix microarrays.

近期研究报告着重指出了诱导多能干细胞 (induced pluripotent stem cells, iPSC) 技术存在的诸多弊端，其中包括移植细胞具备免疫原性的潜在风险，该问题为基于诱导多能干细胞的细胞疗法带来了严峻的安全相关顾虑。不过，支持诱导多能干细胞安全性与有效性的临床前数据也在持续积累。
为解决胚胎干细胞 (embryonic stem cells, ESCs)/诱导多能干细胞 (iPSCs) 或其衍生神经球的免疫原性顾虑，本研究对未分化小鼠胚胎干细胞（EB3细胞系）、小鼠诱导多能干细胞（38C2细胞系）、胚胎干细胞/诱导多能干细胞衍生神经球，以及从胎鼠神经节隆起分离得到的原代培养神经球的全局基因表达谱进行了对比分析。本研究以成年小鼠皮肤成纤维细胞作为对照，采用Affymetrix微阵列 (Affymetrix microarray) 对多种来源制备的神经球的全局基因表达进行了比较。
关键词：通过芯片进行表达谱分析
从神经球中提取的RNA与Affymetrix微阵列进行了杂交。