Single-cell 5' RNA sequencing of Bactrian camel PBMCs

We conducted Single-cell 5' RNA sequencing on PBMCs from four adult healthy camels (C1-C4) from Inner Mongolia. Additionally, two camels (C3, C4) were immunized with BSA as an antigen, and PBMCs were collected at two time points after four immunizations (42 days and 56 days) for single-cell 5' RNA sequencing. We identified cell types in camel PBMCs, focusing on B cell subtypes and differentiation trajectories. Furthermore, we reconstructed single-cell BCR sequences and investigated the associations between B cell phenotypes and BCR types.

本研究对来自内蒙古地区的4只成年健康骆驼（编号C1至C4）的外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMC）开展了单细胞5'端RNA测序。此外，针对其中2只骆驼（C3、C4），以牛血清白蛋白（Bovine Serum Albumin, BSA）作为抗原进行免疫，并在完成4次免疫后的两个时间节点（第42天与第56天）采集其外周血单个核细胞，同样进行单细胞5'端RNA测序。本研究鉴定了骆驼外周血单个核细胞的细胞类型，重点聚焦于B细胞亚型及其分化轨迹；此外还重构了单细胞B细胞受体（B Cell Receptor, BCR）序列，并探究了B细胞表型与BCR类型之间的关联。