RNA-seq of mouse fibroblast-like synoviocytes (FLS) isolated from mice with collagen-induced arthritis (CIA) and naive littermates

Fibroblast-like synoviocytes (FLS) were isolated from the inflamed joints of mice with collagen-induced arthritis (CIA) or the joints of naive littermates to characterise gene expression changes in response to chronic inflammation. Cells were isolated at two times of day (zeitgeber time, ZT4 and ZT16) to characterise diurnal variation in inflammatory symptoms and responses. FLS cells were isolated from joint digests based on Podoplanin expression prior to RNA extraction and library preparation for sequencing.

为表征慢性炎症诱导的基因表达变化，研究人员从胶原诱导性关节炎（collagen-induced arthritis, CIA）模型小鼠的炎症关节，以及未免疫同窝小鼠的关节中分离成纤维样滑膜细胞（fibroblast-like synoviocytes, FLS）。为探究炎症症状与应答的昼夜节律变化，实验选取一天中的两个时间点（授时因子时间zeitgeber time, ZT4与ZT16）开展细胞分离。在进行RNA提取与测序文库制备前，研究人员基于平足蛋白（Podoplanin）的表达水平，从关节消化液中筛选得到FLS细胞。