Gene expression profile analysis of Staphylococcus aureus treated with nisin

Staphylococcus aureus (S. aureus) has already to be one of the most commonly identified bacteria that cause food poisoning. S. aureus colonization in humans can cause serious infections, toxinoses and life threatening diseases. The bacteriocin nisin has been extensively used as potential natural preservative in the food industry, but the overall transcriptional response mechanisms of S. aureus to nisin are still poorly understood. To detect the possible molecular mechanism of nisin against S. aureus, Affymetrix GeneChips were used to determine the global comparative transcription of S. aureus cells triggered by treatment with sub-inhibitory concentrations of nisin. Staphylococcus aureus planktonic cells were exposed for 60 minutes to nisin at concentration of 4 μg/ml (1/2× MIC). 2 samples including 2 control samples are analyzed.

金黄色葡萄球菌（Staphylococcus aureus，简称S. aureus）已然成为最常被检出的食源性致病菌之一。定植于人体的金黄色葡萄球菌可引发严重感染、毒素性疾病乃至危及生命的病症。细菌素乳链菌肽（nisin）已被广泛用作食品工业中极具潜力的天然防腐剂，但目前学界对金黄色葡萄球菌响应乳链菌肽的整体转录调控机制仍知之甚少。为探明乳链菌肽抗金黄色葡萄球菌的潜在分子机制，本研究采用Affymetrix基因芯片（Affymetrix GeneChips），检测经亚抑菌浓度乳链菌肽诱导的金黄色葡萄球菌细胞的全基因组比较转录水平。将浮游态金黄色葡萄球菌置于浓度为4 μg/ml（1/2×最低抑菌浓度，即1/2× MIC）的乳链菌肽环境中处理60分钟，本研究共分析2份处理样本与2份对照样本。