Global changes in gene expression of larval zebrafish. Global changes in gene expression of larval zebrafish

We employed DNA microarray to figure out the possible mechanism of BBR on zebrafish NAFLD model Overall design: AB strain zebrafish (Danio. rerio) were kept under standard laboratory conditions with a 14-h light/10-h dark cycle at a temperature of 28.5±1°C. Embryos and larvae were maintained in embryo medium to 5 dpf without feeding. Zebrafish larvae were fed with control diet (AP100, 30mg/100 fish/day) or high cholesterol diet (HCD, 180mg AP100 plus 4% cholesterol/100fish/day) or HCD plus berberine (5μM) for 10 days.

本研究采用DNA微阵列（DNA microarray）技术，探究小檗碱（Berberine, BBR）对斑马鱼非酒精性脂肪性肝病（Non-Alcoholic Fatty Liver Disease, NAFLD）模型的潜在作用机制。实验设计：AB品系斑马鱼（Danio rerio）在标准实验室条件下饲养，光照周期为14小时光照/10小时黑暗，饲养温度维持在28.5±1℃。胚胎及幼鱼饲养于胚胎培养液中，至受精后5日龄（days post fertilization, dpf），期间不喂食。随后将斑马鱼幼鱼分为三组，分别投喂对照饲料（AP100，30mg/100尾鱼/天）、高胆固醇饲料（High Cholesterol Diet, HCD，180mg AP100添加4%胆固醇/100尾鱼/天），以及高胆固醇饲料联合5μM小檗碱，连续投喂10天。