Ribosome profiling in Arabidopsis T87 cultured cell. Arabidopsis thaliana strain:T87 cultured cells

In gene expression, DNA is transcribed to mRNA, and mRNA is translated to protein. In these steps, translation is one of the important steps to determine the protein abundance. To evaluate translation efficiencies, ribosome profiling is reported in previous study. This method is based on deep sequencing of ribosome-protected mRNA fragments, and provides a "snapshot" of all the ribosomes abundance at a specific time point. This information provide the ribosome position or ribosome occupancy on mRNAs. Thus, we conducted ribosome profiling using 3 day after inoculation of Arabidopsis T87 cultured cell to evaluate translation efficiencies and analyze the relationships between sequence feature and translation process.

在基因表达过程中，脱氧核糖核酸（DNA）经转录生成信使RNA（mRNA），随后mRNA被翻译为蛋白质。在这一系列步骤中，翻译是决定蛋白质丰度的关键环节之一。为评估翻译效率，已有研究采用核糖体谱（ribosome profiling）技术：该技术基于对核糖体保护的mRNA片段的深度测序，可在特定时间点捕捉所有核糖体丰度的“快照”，并反映核糖体在mRNA上的位置及核糖体占据情况。因此，本研究对接种后3天的拟南芥T87培养细胞开展核糖体谱分析，以评估翻译效率，并解析序列特征与翻译过程之间的关联。