Fasting response of mouse brain

Analysis of fasting-induced change of metabolites in mice confirmed that glucose level was reduced in the liver, but unaffected in the brain of fasted mice. To explore molecular mechanisms for the preferential glucose supply to the brain upon fasting, we compared gene expression profiles of the brain between fasted and fed mice. Gene ontology (GO) term analysis revealed the enrichment of one GO term, “active membrane transporters activity”. We also showed that fasting enhances the expression of a glucose transporter Slc2a1 (Glut1) gene. Mice were fasted or fed for 17 hrs, and their brains were dissected for microarray analysis

本研究通过分析禁食诱导的小鼠代谢物变化，证实禁食小鼠肝脏内葡萄糖水平降低，但其脑部葡萄糖水平未受影响。为探究禁食状态下脑部优先获得葡萄糖供给的分子机制，本研究对比了禁食小鼠与正常进食小鼠的脑部基因表达谱。基因本体（Gene Ontology，GO）富集分析显示，GO术语‘主动膜转运蛋白活性’显著富集。本研究同时证实，禁食可上调葡萄糖转运蛋白Slc2a1（Glut1）基因的表达。实验中，小鼠被禁食或正常进食17小时后，取其脑部组织进行微阵列分析。