Small RNA-Seq analysis of human lung fibroblasts exposed to TGF-ß

To assess the transcriptomic response of human lung fibroblast to TGF-ß, human MRC5 pulmonary fibroblasts (CCL-171) were exposed to TGF-ß at 10 ng/mL. RNA samples were harvested 24 hours after TGF-ß exposition. Three independent experiments were carried out. Overall design: 6 samples in triplicates: control versus TGF-ß.

为评估人肺成纤维细胞对转化生长因子-β（TGF-β）的转录组应答，本研究以终浓度10 ng/mL的转化生长因子-β处理人MRC5肺成纤维细胞（CCL-171）。于转化生长因子-β处理24小时后收集RNA样本，共开展3次独立实验。整体实验设计：设置对照组与转化生长因子-β处理组，每组设置3次生物学重复，共计6个样本。