RNA-seq reveals differentially expressed lncRNAs and circRNAs and their associated functional network in HTR-8/Svneo cells under hypoxic conditions

Placental hypoxia is hazardous to maternal health as well as fetal growth and development. Preeclampsia and intrauterine growth restriction are common pregnancy problems, and one of the causes is placental hypoxia.Placental hypoxia is linked to a number of pregnancy illnessesv. To investigate their potential function in anoxic circumstances, we mimicked the anoxic environment of HTR-8/Svneo cells and performed lncRNA and circRNA studies on anoxic HTR-8/Svneo cells using high-throughput RNA sequencing. The miRNA target genes were predicted by integrating the aberrant expression of miRNAs in the placenta of preeclampsia and intrauterine growth restriction, and a ceRNA network map was developed to conduct a complete transcriptomic and bioinformatics investigation of circRNAs and lncRNAs. The signaling pathways in which the genes were primarily engaged were predicted using GO and KEGG analyses. To propose a novel explanation for trophoblastic organism failure caused by lncRNAs and circRNAs in an anoxic environment. Overall design: To investigate their potential function in anoxic circumstances, we mimicked the anoxic environment of HTR-8/Svneo cells and performed lncRNA and circRNA studies on anoxic HTR-8/Svneo cells using high-throughput RNA sequencing. The miRNA target genes were predicted by integrating the aberrant expression of miRNAs in the placenta of preeclampsia and intrauterine growth restriction, and a ceRNA network map was developed to conduct a complete transcriptomic and bioinformatics investigation of circRNAs and lncRNAs.

胎盘缺氧（placental hypoxia）对母体健康与胎儿生长发育均具有危害。子痫前期（preeclampsia）与宫内生长受限（intrauterine growth restriction）为常见妊娠并发症，其诱因之一即为胎盘缺氧。胎盘缺氧与多种妊娠疾病密切相关。为探究长链非编码RNA（long non-coding RNA, lncRNA）与环状RNA（circular RNA, circRNA）在缺氧环境中的潜在功能，我们建立了HTR-8/Svneo细胞的缺氧模型，并通过高通量RNA测序对缺氧状态下的HTR-8/Svneo细胞开展lncRNA与circRNA分析。通过整合子痫前期与宫内生长受限患者胎盘组织中异常表达的微RNA（microRNA, miRNA）预测其靶基因，并构建内源竞争RNA（competing endogenous RNA, ceRNA）调控网络图谱，对circRNA与lncRNA开展全面的转录组学与生物信息学研究。借助基因本体（Gene Ontology, GO）富集分析与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路富集分析，我们预测了上述基因主要参与的信号通路。本研究旨在为缺氧环境下lncRNA与circRNA介导的滋养细胞功能衰竭提供全新的解释视角。总体设计：为探究lncRNA与circRNA在缺氧环境中的潜在功能，我们建立了HTR-8/Svneo细胞的缺氧模型，并通过高通量RNA测序对缺氧状态下的HTR-8/Svneo细胞开展lncRNA与circRNA分析。整合子痫前期与宫内生长受限患者胎盘组织中异常表达的miRNA预测其靶基因，并构建ceRNA调控网络图谱，对circRNA与lncRNA开展全面的转录组学与生物信息学研究。